2022-12-02 阅读(753)

Expression, purification and identification of isotope-labeled recombinant cystatin C protein in Escheichia coli intended for absolute quantification using isotope dilution mass spectrometry

DATE: March 2023           Pubmed ID105785                   IF: 2.205


Isotope-labeled proteins are expected to be used as internal standard proteins in the field of protein quantification by isotope dilution mass spectrometry (ID/MS). To achieve the absolute quantification of Cystatin C (Cys C) based on ID/MS, we aims to obtain 15N isotope-labeled recombinant Cys C (15N-Cys C) protein.


Two human monoclonal SARS-CoV-2 antibodies that maintain neutralizing potency against the SARS-CoV-2 Omicron BA.1 and BA.2 variants

DATE: June 2022            IF: 7.243


The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to sweep the globe with devastating consequences on human lives and world economy. As an RNA virus, SARS-CoV-2 has a relatively high mutation rate and is rapidly evolving.

https://pdf.sciencedirectassets.com/308594/AIP/1-s2.0-S2352304222001623/main.pdf?X-Amz-Security Token=IQoJb3JpZ2luX2


Biflavones inhibit the fibrillation and cytotoxicity of the human islet amyloid polypeptide

DATE: November 2022       IF: 7.571


Biflavones are a kind of natural compound with a variety of biological activities, which have the capability of reversing diabetes and neurodegenerative diseases. The human islet amyloid polypeptide (hIAPP) is closely related to the pathological process of type II diabetes mellitus (T2DM).


Production and characterization of monoclonal antibodies against Toxoplasma gondii ROP18 with strain-specific reactivity

DATE: February 2020       p.p: 940 - 948    


The rhoptry kinase 18 of Toxoplasma gondii (TgROP18) has been identified as a key virulence factor that allows the parasite to escape from host immune defences and promotes its proliferation in host cells. Although much research is focused on the interaction between host cells and TgROP18,the development of monoclonal antibodies (mAbs) against TgROP18 has not been reported till date.


Synthesis of RNA-based gene regulatory devices for redirecting cellular signaling events mediated by p53

DATE: March 2021      Pubmed ID: 33754021     p.p: 4688–4698   IF: 11.556    


Rationale: The p53 gene is a well-known tumor suppressor, and its mutation often contributes to the occurrence and development of tumors. Due to the diversity and complexity of p53 mutations, there is still no effective p53...



Preparation of monoclonal antibody against penicillic acid (PA) and its application in the immunological detection

DATE: July 2020          Pubmed ID: 126505                IF: 9.231


The high content of Penicillic acid (PA) in the feed pose threat to human health and cause serious losses to economic wealth through the enrichment effect of the food chain. 



ALG3 contributes to the malignancy of non-small cell lung cancer and is negatively regulated by MiR-98-5p

DATE: March 2020         Pubmed ID: 152761                 IF: 3.309


Alpha-1,3-mannosyltransferase (ALG3) is an oncoprotein associated with multiple malignancies. We aimed to investigate the role and potential mechanisms of ALG3 in non-small cell lung cancer (NSCLC).




Purification and Characterization of Antioxidant Peptides from Alcalase-Hydrolyzed Soybean (Glycinemax L.) Hydrolysate and Their Cytoprotective Effects in Human Intestinal Caco-2 Cells

DATE: May 2019            p.p: 5772–5781


This study aimed to purify and identify antioxidant peptides from the low-molecular-weight fraction (SPH-I, MW < 3 kDa) of 

Alcalase-hydrolyzed soybean (Glycinemax L.) hydrolysate and further evaluate the cytoprotective effects of synthesized

 peptides against oxidative stress in human intestinal Caco-2 cells.


Expression and Mechanism of Interleukin 1 (IL-1), Interleukin 2 (IL-2), Interleukin 8 (IL-8), BMP, Fibroblast Growth Factor 1 (FGF1), and Insulin-Like Growth Factor (IGF-1) in Lumbar Disc Herniation

DATE: February 2019      Pubmed ID: 30716059       p.p: 984–990   


The expression and mechanism of IL-1, IL-2, IL-8, BMP, FGF1, and IGF-1 in Sprague-Dawley (SD) rats with lumbar disc herniation were investigated.



Novel mono-lipidated dimeric glucagon-like peptide-1 receptor agonist with improved long-acting and hypoglycemic activity

DATE: January 2019        p.p: 9654-9662


Dimerization is a useful tool to boost ligand–receptor interaction. Both lipidation and dimerization effectively prolong the short half-life (t1/2) of peptides by facilitating binding with serum albumin and increasing hydrodynamic size.




Ultrasensitive analysis of heat shock protein 90α with antibodies orderly arrayed on a novel type of immunoprobe based on magnetic COFs

DATE: January 2019        p.p: 553-560                  IF: 6.556


The early diagnosis of liver cancer by target biomarkers is of great significance for improving the survival rate of cancer patients. However, it is still a challenging task to sensitively detect circulating protein biomarkers due to decreased binding activity of antibodies originating from uncontrolled orientation of immobilization on the surface of a solid matrix.



Mitochondrial Drp1 recognizes and induces excessive mPTP opening after hypoxia through BAX-PiC and LRRK2-HK2

DATE: November 2021       IF: 9.685


Mitochondrial mass imbalance is one of the key causes of cardiovascular dysfunction after hypoxia. The activation of dynaminrelated protein 1 (Drp1), as well as its mitochondrial translocation, play important roles in the changes of both mitochondrial morphology and mitochondrial functions after hypoxia. However, in addition to mediating mitochondrial fifission, whether Drp1 has other regulatory roles in mitochondrial homeostasis after mitochondrial translocation is unknown.



Novel Angiotensin-Converting Enzyme Inhibitory Peptides Identified from Walnut Glutelin-1 Hydrolysates: Molecular Interaction, Stability, and Antihypertensive Effects

DATE: November 2021       IF: 6.706


Abstract:In recent years, angiotensin-converting enzyme (ACE) inhibitory peptide has become a research hotspot because

of its essential role in maintaining human blood pressure balance. In this study, two novel ACE inhibitory peptides of Val-

Glu-Arg-Gly-Arg-Arg-lle-Thr-Ser-Val (Valine-Glutamate-Arginine-Glycine-Arginine-Arginine-Isoleucine-Threonine-Serine-

Valine,VERGRRITSV)and Phe-Val-Ile-Glu-Pro-Asn-Ile-Thr-Pro-Ala (Phenylalanine-Valine-Isoleucine-Glutamate-Proline-

Asparagine-Isoleucine-Threonine-Proline-Alanine, FVIEPNITPA) were isolated and purified from defatted walnut meal 

hydrolysates through a series of preparation processes including ultrafiltration, Sephadex G-15 gel chromatography, and 

reverse high performance liquid chromatography (RP-HPLC). Both peptides showed high ACE inhibitory activities.


血清 Hcy、BNP、IL-8 在冠心病患者中的表达及意义



摘要:目的 探讨血清同型半胱氨酸( Hcy) 、B 型利钠肽( BNP) 、白细胞介素-8( IL-8) 在冠心病患者中的表达及意义。方法选取 2016年1月至2018年12月本院收治的冠心病患者 200例为研究组,健康志愿者80例为对照组。





摘要: 目的 研究淡水鲈鱼小清蛋白(parvalbumin, PV)的致敏性, 同时探索其对小鼠肠道菌群的影响。方法 36 只 C3H/HeJ 小鼠按体重随机分为 3 组, 分别为空白对照组(CK 组)、卵清白蛋白(ovalbumin, OVA)致敏对照组(OVA 组)和小清蛋白致敏组(PV 组)。






摘要:目的 探讨大鼠脑缺血性神经损伤后脑红蛋白( NGB) 对受损神经元轴突生长能力的调控作用及其机制。方法将 30 只 SD 大鼠随机分为假手术组、模型组、Hemin 组,每组 10 只,模型组、Hemin 组采用双侧颈总动脉结扎的方法制备脑缺血模型,假手术组只暴露双侧颈总动脉但不穿线结扎。





摘  要:为进一步了解微生物凝乳酶的结构特性,根据GenBank数据库中甲醇芽孢杆菌凝乳酶(I3EB99)的氨基酸序列和大肠杆菌密码子的偏爱性,设计合成此凝乳酶的全基因序列,构建原核表达载体,通过BL21(DE3)表达其融合蛋白,将获得的融合蛋白进行His标签特异性亲和纯化,并利用生物信息学方法研究凝乳酶的三维空间立体结构。