卡梅德生物科技（天津）有限公司
400-621-6806
服务电话：400-621-6806
地址：天津市武清区国际企业社区A5号楼五层，301700
邮箱：info@kmdbioscience.com
| 产品编号 | COV444 |
|---|---|
| 产品名称 | Recombinant SARS-CoV-2/COVID-19 Omicron Spike RBD Protein, His tag |
| 规格 | 100ug |
| 物种 | COVID-19 |
| 宿主 | HEK293 Cells |
| 内毒素水平 | Less than 1.0 EU per μg by the LAL method. |
| 生物活性 | Recombinant SARS-CoV-2/COVID-19 Omicron Spike RBD Protein (COV444) can bind human ACE2 protein. |
| 缓冲体系 | Lyophilized from sterile PBS, pH 7.4. |
| 纯化方式 | Affinity purification |
| 产品背景 | A novel severe acute respiratory syndrome (SARS)-like coronavirus (SARS-CoV-2) emerged and is rapidly spreading in humans, causing COVID-19. A key to tackling this pandemic is to understand the receptor recognition mechanism of the virus, which regulates its infectivity, pathogenesis and host range. SARS-CoV-2 and SARS-CoV recognize the same receptor—angiotensin-converting enzyme 2 (ACE2)—in humans. Researchers determined the crystal structure of the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 (engineered to facilitate crystallization) in complex with ACE2. In comparison with the SARS-CoV RBD, an ACE2-binding ridge in SARS-CoV-2 RBD has a more compact conformation; moreover, several residue changes in the SARS-CoV-2 RBD stabilize two virus-binding hotspots at the RBD–ACE2 interface. These structural features of SARS-CoV-2 RBD increase its ACE2-binding affinity. Additionally, we show that RaTG13, a bat coronavirus that is closely related to SARS-CoV-2, also uses human ACE2 as its receptor. |
| 保存条件 | Aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles. |
津公网安备12011402001522号
