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| 产品编号 | PA180 |
|---|---|
| 产品名称 | Human respiratory syncytial virus Fusion Protein, His Tag |
| 产品别称 | F Protein, RSV,HRSVgp08 Protein, RSV |
| 产品描述 | A DNA sequence encoding the human respiratory syncytial virus Fusion Protein (Met1-Ile525) was expressed with a polyhistidine tag at the C-terminus. |
| 分子名称 | RSV-F |
| 规格 | 100ug |
| 物种 | RSV |
| 宿主 | Baculovirus-Insect Cells |
| 分子量 | 57.12 kDa, AA 1-525 |
| 标签 | His |
| 纯度 | > 90 % as determined by SDS-PAGE. |
| 内毒素水平 | < 1.0 EU per μg protein as determined by the LAL method. |
| 缓冲体系 | Lyophilized from sterile 20mM PB, pH 7.5, 300mM NaCl, 10% glycerol. |
| 纯化方式 | 亲和纯化 |
| 产品背景 | Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis. |
| 保存条件 | Store it under sterile conditions at -80℃. |
| 运输条件 | 冰袋运输 |
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